Reporter

Part:BBa_K5005000:Design

Designed by: YunQi Chen   Group: iGEM23_GEC-CHINA   (2023-10-05)


EBFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In target plasmid, BFP is located downstream of the T7 promoter (derived from site-directed mutagenesis of EGFP), expressing the target gene BFP (blue fluorescence), which could potentially be evolved back into EGFP by Editor.

By transforming the EGFP (BBa_K5005001), the target BFP fragment is obtained. More details about primers is on the page of BBa_K5005001.

Source

Constructed in this work.

References